Biopharmaceutical Applications

1. Amino acids

In the production of intermediates, raw materials, and other biological products using biological fermentation as the main production process, there are numerous mycelium, proteins, residual sugars, pigments, inorganic ions, and other culture medium residues in the feed solution that affect production control and product quality. Currently, most of the fermentation liquid filtration processes in China still use traditional separation equipment such as plate frames, drums, centrifuges, or methods such as flocculation settling, centrifugation, and isoelectric crystallization. However, these traditional methods can only roughly separate solid substances such as mycelium and large suspended solids in the fermentation broth, and cannot remove small molecular impurities such as soluble proteins, impurities, and pigments that are present in the fermentation broth; The presence of these small molecule impurities will greatly increase the load of subsequent refining processes such as dissociation, solvent extraction, decolorization, crystallization, and evaporation concentration, leading to problems such as large wastewater discharge, increased energy consumption, and unstable product quality.

Application object:

Antibiotics: cephalosporins, colistin sulfate, streptomycin sulfate, erythromycin, vancomycin, streptomycin, amphotericin, lincomycin, spectinomycin, etc

Amino acids: lactic acid, citric acid, itaconic acid, succinic acid and other basic acids: lysine, glutamic acid, phenylalanine, threonine, tryptophan, glycine, arginine, glutamine, theanine, proline, valine, isoleucine, etc

Vitamins: Vitamin C, Vitamin B2. Vitamin B12. etc

Enzyme preparations: amidase, phytase, rennet, mannanase, papain, dehydrogenase, etc

Nucleosides: inosine, guanosine, cytidine, phosphatidylcholine, etc

Pharmaceutical intermediates: statins, prills, D-hydroxyphenylglycine, aminobutyric acid, mandelic acid, etc

Bulk chemical products: propylene glycol, glycerol, acrylamide, etc

Other food additives: soy sauce, nisin, natamycin, essence and spices

Process characteristics:

1. Compared with traditional separation processes, membrane separation has high accuracy, uniform pore size distribution, and guaranteed filtrate quality; The membrane surface is smooth, the membrane flux decay rate is slow, and it can maintain high-throughput filtration with reduced cleaning frequency

2. Improve product yield, reduce wastewater volume, diversify pore size, suitable for processing requirements of different types of materials

3. No need to add additives, concentrated substances (mycelium, protein, etc.) can be recycled as feed

4. Can ensure the integrity and activity of over 95% of the fermented bacterial cells

5. Organic ultrafiltration membrane removes small molecule proteins or peptides, nucleic acids, pigments and other impurities from ceramic membrane clear solution, improves product purity and reduces sewage treatment load

6. The desalination and pre concentration of the target product can be achieved, reducing the production load of subsequent processing processes such as solvent extraction, separation, and evaporation concentration

2. Antibiotics

This technology is suitable for modern antibiotic industrial production using microbial synthesis methods and can replace traditional refining techniques such as adsorption, precipitation, solvent extraction, ion exchange, etc. The separation process has no chemical reactions, no phase changes, no damage to biological activity, and is environmentally friendly. It has been successfully applied in antibiotic production enterprises such as cephalosporins, erythromycin, vancomycin, as well as enzyme preparations, pharmaceutical intermediates, and other food additive production enterprises.

In the production of antibiotics (cephalosporins, streptococci, penicillins, erythromycin, etc.), organic acids (lysine, glutamic acid, L-lactic acid citric acid, nucleotides, etc.), enzyme preparations (phytic acid plum, etc.), and other pharmaceutical and edible products, the use of ceramic membrane ultrafiltration technology to replace traditional filtration processes such as plate frame, drum, centrifuge, diatomaceous earth, etc. for the removal of bacterial cells and macromolecules from fermentation broth has the following outstanding advantages:

1. The yield of active ingredients is high, which is 5-12% higher than using traditional filtration methods;

2. High separation accuracy, low impurity content in the permeate, clear and transparent, reducing the difficulty of subsequent processing;

3. High concentration factor, greatly reducing water usage and reducing wastewater discharge;

4. Long continuous working time, easy and efficient regeneration, with a cost of 1/5~1/10 of organic film;

5. Membrane components have a long service life, which is 3-10 times that of organic membranes;

6. The service life of supporting ion exchange resin and macroporous adsorption resin can be extended by 2-3 times;

7. Fully automatic control, combining semi-automatic and manual systems, with low labor intensity;

8. The process setting is professional and meets FDA and GMP requirements.

9. The performance price ratio is higher than any effective separation method.

10. Supporting nanofiltration concentration to form a membrane integrated system;

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