Extraction of cephalosporin C

Cephalosporin C is the starting material for semi synthetic cephalosporin antibiotics. At present, fermentation is used for production, and impurities such as mycelium, protein, and suspended particles are often present in the fermentation broth. The current process is to use a two-step separation method of plate and frame pressure filtration or vacuum drum filtration to remove these impurities after pretreatment of the fermentation broth. The operation process is tedious, the filtrate quality is not high, and the yield is low. in recent years. Membrane separation technology, as a new type of membrane separation technology, has achieved good results in the extraction of various antibiotics and has been widely applied.

Membrane separation technology is suitable for modern antibiotic industrial production using microbial synthesis methods and can replace traditional refining techniques such as adsorption, precipitation, solvent extraction, ion exchange, etc. The separation process has no chemical reactions, no phase changes, no damage to biological activity, and is environmentally friendly. It has been successfully applied in antibiotic production enterprises such as cephalosporins, erythromycin, vancomycin, enzyme preparations, pharmaceutical intermediates, and other food additive production enterprises. Ceramic membrane ultrafiltration technology is used to replace traditional filtration processes such as plate frame, rotary drum, centrifugation, and diatomaceous earth for the removal of bacterial cells and macromolecules from fermentation broth. After concentration through nanofiltration membrane, the amount of extractant used is greatly reduced and the production capacity of the equipment is improved.

The advantages of membrane separation technology in extracting cephalosporin C

1. System cross flow operation design, no need to add filter aids, will not introduce new impurities, completely solving the problem of pollution blockage;

2. High separation accuracy, high transmittance of filtrate, reduced pollution from ion exchange resin and vacuum concentration, and promoted crystallization;

3. Conducted at room temperature, with mild conditions and no component damage, especially suitable for heat sensitive substances;

4. It can achieve fully automatic control of PLC upper computer, with simple operation, greatly reducing labor intensity, and easy to clean and maintain;

5. It can achieve high fold concentration and significantly improve product yield (5-12%) compared to traditional processes. The concentrated bacterial cells can be recycled as feed;

6. All pipelines in contact with the extraction solution are made of materials that meet hygiene requirements and comply with GMP and other certification standards.