Integrated process of antibiotic fermentation liquid membrane

Antibiotics refer to a class of secondary metabolites produced by microorganisms (including bacteria, fungi, actinomycetes) or higher animals and plants in their daily lives, which have resistance to pathogens or other activities, and can interfere with the developmental functions of other living cells. The commonly used antibiotics in clinical practice include extracts from microbial culture media and compounds synthesized or semi synthesized by chemical methods. With the development of the antibiotic industry, the preparation of antibiotics has received widespread attention. The traditional process of refining antibiotics is not only cumbersome and low in yield, but also affects the quality of the product. Membrane technology, as a new type of separation technology, has been applied to the concentration of antibiotic fermentation broth, greatly improving the yield and quality of the product.

Taking antibiotics prepared by fermentation method as an example, the fermentation broth has a large volume and a low content of antibiotics (usually only about 0.1-5% of the fermentation broth volume, and some new antibiotics have even lower concentrations). After pre-treatment and ceramic ultrafiltration membrane filtration in the early stage, whether using resin separation or phase exchange refining, the obtained drug solution has a large volume, low content, and high energy consumption for direct concentration. If thermal concentration is used, it may also lead to the thermal decomposition of antibiotics. Based on the situation of antibiotic fermentation broth and the characteristics of membrane separation technology, Shengchuan Membrane Separation has developed a membrane integrated process system for antibiotic fermentation broth. Membrane technology is used to filter and concentrate the fermentation broth at room temperature or low temperature, greatly improving the yield and quality of the product.

Integrated process of antibiotic film

The relative molecular weight of antibiotics is mostly in the range of 300-1200. Membrane integration technology can improve the filtration, concentration, and purification processes of antibiotics from three aspects:

1. After pretreatment, antibiotic fermentation broth is filtered using ceramic ultrafiltration membrane for impurity removal, which can improve the quality of antibiotic filtrate and increase the recovery rate of antibiotics (up to 95%).

2. Nanofiltration membrane concentrates the unextracted antibiotic fermentation filtrate, removes water and inorganic salts, and then extracts. This can significantly improve the production capacity of the equipment and greatly reduce the amount of extractant used.

3. After solvent extraction of antibiotics, the extract is concentrated using a solvent resistant nanofiltration membrane, and the penetrating extractant can be recycled.

Advantages of antibiotic membrane separation equipment process

1. The antibiotic membrane separation equipment has high separation accuracy;

2. Wide applicability and resistance to strong polar solvents;

3. System cross flow operation design, no need to add filter aids, will not introduce new impurities, completely solving the problem of pollution blockage;

4. It can achieve high fold concentration and significantly improve product yield compared to traditional processes. The concentrated bacterial cells can be recycled as feed;

5. It can achieve fully automatic control of PLC upper computer, with simple operation, greatly reducing labor intensity, and easy to clean and maintain;

6. The significant reduction in wastewater discharge and COD promotes the realization of clean production.