Process of Pineapple Proteinase Concentration and Separation Equipment

Pineapple protease concentration and separation equipment process Pineapple protease is widely present in the fruit, buds, leaves, and stems of pineapples. It is extracted from the fruit of pineapples to become fruit bromelain, which is extracted from the stems of pineapples

Process of Pineapple Proteinase Concentration and Separation Equipment

Pineapple protease is widely present in the fruit, buds, leaves, and stems of pineapples. It is extracted from the fruit of pineapples as fruit bromelain and from the stem of pineapples as stem bromelain. Pineapple protease has a molecular weight of 33kDa and is a typical thiol protease that can decompose proteins, peptides, esters, and amides. Its activity in hydrolyzing proteins is more than 10 times higher than that of papain, making it widely used. It can be used as a food additive for meat tenderization, beer clarification, as well as for the production of cheese, gelatin, and hydrolyzed proteases. There are three traditional processes for extracting bromelain: organic solvent precipitation, tannin precipitation, and kaolin adsorption. Although these three methods have simple production processes and low investment, the process cycle is long and pineapple juice is prone to spoilage. Moreover, tannins and organic solvents can denature enzymes, resulting in low product activity; The production process and operation of kaolin adsorption method are very complex, with high raw material consumption, low enzyme activity and overall yield, and the origin of kaolin has a significant impact on the adsorption method.

The main process operations for extracting bromelain by ultrafiltration include the following steps:

Squeezing: Wash the pineapple stem, press out the juice with a press machine, add 0.05% sodium benzoate for preservation, and quickly filter out impurities through snail and dish separation;

Ultrafiltration separation and concentration: Concentrate and separate the clarified juice in an ultrafiltration device, so that the volume is concentrated to 1/5 of the original volume;

Precipitation: Cool the concentrated solution to 0-4 ℃, add 95% ethanol at -20 ℃ while stirring until the ethanol concentration in the mixture reaches 50%, let it stand to precipitate the enzyme, remove the supernatant, and obtain wet enzyme;

Drying: Wet enzymes are dried under reduced pressure at 0 ℃ or freeze-dried under vacuum to obtain high-quality bromelain.