Vancomycin extraction membrane separation technology

Vancomycin is an effective and relatively safe antibiotic for the treatment of severe infections caused by methicillin-resistant Staphylococcus aureus, methicillin-resistant coagulase negative Staphylococcus aureus, and enterococci, including sepsis, pulmonary infections, and skin and soft tissue infections. Vancomycin is the preferred drug for hospital acquired pulmonary infections caused by methicillin-resistant Staphylococcus aureus. At present, traditional filtration processes are widely used to purify vancomycin both domestically and internationally, which have problems such as low filtration yield, insufficient filtration accuracy, and cumbersome operation processes. Membrane separation technology is widely used in the separation and purification of antibiotics due to its characteristics of no phase change and simple operation.

The fermentation broth of vancomycin contains a large amount of other impurities. These impurities are some of the mycelium itself, some of the unused culture medium, some of the other metabolites produced by the bacteria, and some of the substances that need to be added during the pre-treatment process of the fermentation broth. The concentration of these impurities in the fermentation broth often exceeds that of antibiotics by hundreds, thousands, or even tens of thousands of times. Moreover, many of the metabolites have physical and chemical properties that are very similar to antibiotics, and even have the same chemical composition as antibiotics, only differing in their three-dimensional configurations.

Membrane separation technology is suitable for modern antibiotic industrial production using microbial synthesis methods and can replace traditional refining techniques such as adsorption, precipitation, solvent extraction, ion exchange, etc. The separation process has no chemical reactions, no phase changes, no damage to biological activity, and is environmentally friendly. It has been successfully applied in antibiotic production enterprises such as cephalosporins, erythromycin, vancomycin, as well as enzyme preparations, pharmaceutical intermediates, and other food additive production enterprises. Ceramic membrane ultrafiltration technology is used to replace traditional filtration processes such as plate frame, rotary drum, centrifugation, and diatomaceous earth for the removal of bacterial cells and macromolecules from fermentation broth. After concentration through nanofiltration membrane, the amount of extractant used is greatly reduced and the production capacity of the equipment is improved.

Advantages of Vancomycin Extraction Membrane Separation Technology:

1. High separation accuracy, clear and transparent filtrate, low impurity content, greatly reducing the difficulty of subsequent processing;

2. Separation at room temperature, suitable for the separation, concentration, and purification of thermosensitive substances such as drugs, fruits, enzymes, etc;

3. By using a specially designed operating process, impurities are not easily blocked on the surface of the membrane, solving the problem of membrane fouling and blockage. The membrane permeate water can be recycled and reused in the pre extraction process, reducing environmental pressure;

4. It can achieve high fold concentration and significantly improve product yield (5-12%) compared to traditional processes. The concentrated bacterial cells can be recycled as feed;

5. The membrane production equipment adopts modular design and frequency converter control, with high degree of automation and low labor intensity.